Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation | |
Zhang, Ruili1; Wang, Zhe4; Yang, Zuo1; Wang, Linlin1; Wang, Zhongdi1; Chen, Baihang1; Wang, Zhongliang1; Tian, Jie1,2,3 | |
发表期刊 | THERANOSTICS |
ISSN | 1838-7640 |
2019 | |
卷号 | 9期号:18页码:5386-5395 |
通讯作者 | Wang, Zhongliang(wangzl@xidian.edu.cn) ; Tian, Jie(tian@ieee.org) |
摘要 | To achieve the clinical potential of neural stem cells (NSCs), it is crucial to activate NSC differentiation into neurons and simultaneously monitor the process of NSC differentiation. However, there are many challenges associated with regulating and tracking NSC differentiation. Methods: We developed a redox-responsive multifunctional nanocomplex with a disulfide bond-cvNC-for the delivery of siRNAs to induce NSC differentiation through sequence-specific RNA interference (RNAi) and real-time imaging of sequential mRNA expression during differentiation. The stability and specificity of cvNCs were studied in vitro. Controlled release of siRNA, gene silencing efficiency, as well as real-time imaging of cvNCs on Tubb3 and Fox3 mRNAs during NSC differentiation were evaluated. Results: The introduction of a redox-sensitive disulfide bond not only ensures the remarkable performance of cvNC, such as high stability, controlled siRNA release, and enhanced gene silencing efficiency, but also effectively stimulates NSC differentiation into neurons. More importantly, the cvNC can track NSC differentiation in real-time by monitoring the sequential expression of mRNAs. Conclusion: Our study indicates that cvNC can serve as a robust system for exploring NSCs differentiation process as well as other biological events in living cells. |
关键词 | gold nanoparticle gene silencing imaging drug delivery neural stem cell |
DOI | 10.7150/thno.35032 |
关键词[WOS] | SIRNA DELIVERY ; GOLD NANOPARTICLES ; CANCER ; RESISTANCE ; PROGRESS ; DISEASE ; ACID |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2017YFC1309100] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81671753] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[21804104] ; National Natural Science Foundation of China[81501524] ; National 1000 Young Talents Program of China ; Natural Science Basic Research Plan in Shaanxi Province of China[2019JQ-139] ; Natural Science Basic Research Plan in Shaanxi Province of China[2018JM2041] ; Fundamental Research Funds for the Central Universities[JB191211] ; Fundamental Research Funds for the Central Universities[JB191207] ; Fundamental Research Funds for the Central Universities[JB191208] ; National Key Research and Development Program of China[2017YFC1309100] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81671753] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[21804104] ; National Natural Science Foundation of China[81501524] ; National 1000 Young Talents Program of China ; Natural Science Basic Research Plan in Shaanxi Province of China[2019JQ-139] ; Natural Science Basic Research Plan in Shaanxi Province of China[2018JM2041] ; Fundamental Research Funds for the Central Universities[JB191211] ; Fundamental Research Funds for the Central Universities[JB191207] ; Fundamental Research Funds for the Central Universities[JB191208] |
项目资助者 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; National 1000 Young Talents Program of China ; Natural Science Basic Research Plan in Shaanxi Province of China ; Fundamental Research Funds for the Central Universities |
WOS研究方向 | Research & Experimental Medicine |
WOS类目 | Medicine, Research & Experimental |
WOS记录号 | WOS:000474899500018 |
出版者 | IVYSPRING INT PUBL |
七大方向——子方向分类 | 其他 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ia.ac.cn/handle/173211/26874 |
专题 | 中国科学院分子影像重点实验室 |
通讯作者 | Wang, Zhongliang; Tian, Jie |
作者单位 | 1.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuroimaging, Xian 710126, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China 3.Beihang Univ, Sch Med, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100191, Peoples R China 4.Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, NIH, Bethesda, MD 20892 USA |
通讯作者单位 | 中国科学院自动化研究所 |
推荐引用方式 GB/T 7714 | Zhang, Ruili,Wang, Zhe,Yang, Zuo,et al. RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation[J]. THERANOSTICS,2019,9(18):5386-5395. |
APA | Zhang, Ruili.,Wang, Zhe.,Yang, Zuo.,Wang, Linlin.,Wang, Zhongdi.,...&Tian, Jie.(2019).RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation.THERANOSTICS,9(18),5386-5395. |
MLA | Zhang, Ruili,et al."RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation".THERANOSTICS 9.18(2019):5386-5395. |
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