SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy

	SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy
	Ma, Xibo1 ; Cheng, Zhen 2; Jin, Yushen3 ; Liang, Xiaolong4 ; Yang, Xin1 ; Dai, Zhifei 4; Tian, Jie 1
发表期刊	BIOMATERIALS
	2014-03-01
卷号	35 期号:9 页码:2878-2889
文章类型	Article
摘要	SM5-1 is a humanized mouse antibody which has a high binding specificity for a membrane protein of about 230 kDa overexpressed in hepatocellular carcinoma (HCC), melanoma and breast cancer. In this study, SM5-1-conjugated poly D, I. (lactide-coglycolide) (PLA) PLA containing Cy7 (PLA-Cy7-SM5-1) was prepared to study the targeting specificity of the bioconjugate to HCC-LM3-fLuc cell. Then, SM5-1-conjugated PLA containing 5-fluorouracil (5-FU) (PLA-5FU-SM5-1) and PLA containing 5-FU (PLA-5FU) were prepared for treatment of subcutaneous HCC-LM3-fLuc tumor mice. The results showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 45.07%, 23.56% and 19.05% tumor growth inhibition rate, respectively, on day 31 post-treatment as determined by bioluminescent intensity. In addition, in order to evaluate the antitumor efficacy of PLA-5FU-SM5-1, HCC-LM3-fLuc cells were injected into the liver to establish the experimental orthotopic liver tumor models. The experiments showed that PLA-5FU-SM5-1, PLA-5FU and 5-FU induced a 53.24%, 31.00%, and 18.11% tumor growth inhibition rate, respectively, on day 31 post-treatment determined by the bioluminescent intensity of the abdomen in tumor-bearing mice. Furthermore, we have calculated the three-dimensional location of the liver cancer in mice using a multilevel adaptive finite element algorithm based on bioluminescent intensity decay calibration. The reconstruction results demonstrated that PLA-5FU-SM5-1 inhibited the tumor rapid progression, which were consistent with the results of subcutaneous tumor mice experiments and in vitro cell experiment results. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
关键词	Nanoparticles Bioluminescence Imaging (Bli) Bioluminescent Tomography (Blt) Targeted Imaging And Therapy
WOS标题词	Science & Technology ; Technology
关键词[WOS]	PROTEIN-PROTEIN INTERACTIONS ; POSITRON-EMISSION-TOMOGRAPHY ; GROWTH-FACTOR RECEPTOR ; BIOLUMINESCENCE TOMOGRAPHY ; IN-VIVO ; BREAST-CANCER ; MONOCLONAL-ANTIBODY ; 1ST-LINE TREATMENT ; COLORECTAL-CANCER ; TUMOR-METASTASIS
收录类别	SCI
语种	英语
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000332188900031
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/4033
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie
作者单位	1.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China 2.Stanford Univ, Dept Radiol, MIPS, BioX Program, Stanford, CA 94305 USA 3.Harbin Inst Technol, Sch Life Sci & Technol, Nanomed & Biosensor Lab, Harbin 150080, Peoples R China 4.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Ma, Xibo,Cheng, Zhen,Jin, Yushen,et al. SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy[J]. BIOMATERIALS,2014,35(9):2878-2889.
APA	Ma, Xibo.,Cheng, Zhen.,Jin, Yushen.,Liang, Xiaolong.,Yang, Xin.,...&Tian, Jie.(2014).SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy.BIOMATERIALS,35(9),2878-2889.
MLA	Ma, Xibo,et al."SM5-1-Conjugated PLA nanoparticles loaded with 5-fluorouracil for targeted hepatocellular carcinoma imaging and therapy".BIOMATERIALS 35.9(2014):2878-2889.

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