CASIA OpenIR  > 中国科学院分子影像重点实验室
Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging
Wang, Qian1,2; Yan, Hao3,4; Wang, Zihua5; Li, Zhangfu6; Li, Dan6; Li, Zheng7; Wang, Kun2; Tian, Jie2; Zhao, Xinming1
Source PublicationBIOMATERIALS
ISSN0142-9612
2020-10-01
Issue255Pages:120161
Abstract

Early detection and diagnosis are the most important endeavors for reducing associated morbidity and mortality of pancreatic ductal adenocarcinoma (PDAC). Developing molecular imaging probes that can specifically and effectively target cancer-associated biological pathways is one of the key points for sensitive and accurate diagnosis for PDAC. Herein, a small-sized, bispecific fusion protein constructed by genetic fusion of different binding domains of antibodies, termed Bi50, with enhanced targeting effect for PDAC is reported. Bi50 has excellent bispecific targeting for vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) simultaneously in vitro and in vivo. Additionally, Bi50 shows increased intratumoral permeability and enrichment characteristics in the tumor than the control protein, which is constructed directly connecting two individual Fabs. Moreover, Bi50 can not only target areas rich in vasculature but also bind with affinity to tumor parenchymal cells, achieving "multilevel" targeting effect. Our work demonstrates that the bispecific fusion protein Bi50 has great potential as an efficient, targeted molecular imaging probe.

KeywordBispecific targeting Fusion protein Antibody Molecular imaging Pancreatic cancer
DOI10.1016/j.biomaterials.2020.120161
WOS KeywordEGFR ; INHIBITORS ; SURVIVAL ; EFFICACY ; BARRIERS ; SURFACE ; VEGFR
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81,901,813] ; National Natural Science Foundation of China[81,671,757] ; National Natural Science Foundation of China[81,527,805] ; National Natural Science Foundation of China[81,801,766] ; National Natural Science Foundation of China[61,671,449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-I2M-1-001]
Funding OrganizationNational Natural Science Foundation of China ; National Key Research and Development Program of China ; CAMS Innovation Fund for Medical Sciences
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000555693800016
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/40377
Collection中国科学院分子影像重点实验室
Corresponding AuthorWang, Kun; Tian, Jie; Zhao, Xinming
Affiliation1.Chinese Acad Med Sci & Peking Union Med Coll, Dept Diagnost Imaging, Natl Canc Ctr, Canc Hosp, Beijing 100021, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Mol Imaging, Inst Automat, Beijing 100190, Peoples R China
3.Harvard Med Sch, 50 Blossom St, Boston, MA 02114 USA
4.Massachusetts Gen Hosp, Wellman Ctr Photomed, 50 Blossom St, Boston, MA 02114 USA
5.Fujian Med Univ, Sch Basic Med Sci, Fuzhou 350108, Fujian, Peoples R China
6.Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Mol Oncol, Natl Canc Ctr, Canc Hosp, Beijing 100021, Peoples R China
7.Yi Chuang Inst Biotechnol Ind, Beijing 101111, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qian,Yan, Hao,Wang, Zihua,et al. Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging[J]. BIOMATERIALS,2020(255):120161.
APA Wang, Qian.,Yan, Hao.,Wang, Zihua.,Li, Zhangfu.,Li, Dan.,...&Zhao, Xinming.(2020).Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging.BIOMATERIALS(255),120161.
MLA Wang, Qian,et al."Construction of a novel bispecific fusion protein to enhance targeting for pancreatic cancer imaging".BIOMATERIALS .255(2020):120161.
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