Knowledge Commons of Institute of Automation，CAS
| Fancb deficiency causes premature ovarian insufficiency in mice(dagger) | |
| Cen, Changhuo1,2; Chen, Junhua4; Lin, Limei5; Chen, Min1,2,3,6; Dong, Fangfang1,2,6; Shen, Zhiming1,2,6; Cui, Xiuhong1,2; Hou, Xiaohui4; Gao, Fei1,2,3,6 | |
| 发表期刊 | BIOLOGY OF REPRODUCTION
 |
| ISSN | 0006-3363 |
| 2022-05-20 | |
| 页码 | 10 |
| 通讯作者 | Hou, Xiaohui(hxh19801122@163.com) ; Gao, Fei(gaof@ioz.ac.cn) |
| 摘要 | Fancb plays an essential role in the maintenance of germ cell development in female gonads and is a potentially causative gene for premature ovarian insufficiency. Fanconi anemia complementation group B (FANCB) protein is a major component of the Fanconi anemia (FA) core complex and plays an important role in hematopoiesis and germ cell development. Deletion of Fancb gene causes the defect of primordial germ cell (PGC) development and infertility in male mice. However, it remains unknown whether Fancb is required for female germ cell development. In this study, we found that the fertility of Fancb knockout male mice in C57/ICR mixed backgrounds was not affected. Female Fancb(-/-) mice were obtained by crossing Fancb(+/-) females with Fancb(-/Y) males. The number of PGCs was dramatically decreased in Fancb(-/-) females. Very few oocytes were observed after birth and the primordial follicle pool was completely depleted at 6 weeks of age in Fancb(-/-) females. However, the remained oocytes from Fancb(-/-) mice were normal in fertilization and embryonic development from 2-cell to the blastocyst stage. We also found that Fancb and Fancl double-knockout males were also fertile and the number of sperm in epididymis was not reduced as compared to that of Fancb(-/-) and Fancl(-/-) single-knockout mice. Taken together, these results showed that Fancb is also essential for female germ cell development. Inactivation of Fancb causes massive germ cell loss and infertility in adult females. We also found that Fancb and Fancl do not act synergistically in regulating germ cell development. |
| 关键词 | Fancb Fancl primordial germ cells (PGCs) spermatogonial stem cells (SSCs) premature ovarian insufficiency (POI) |
| DOI | 10.1093/biolre/ioac103 |
| 关键词[WOS] | ANEMIA PATHWAY ; GROUP-C ; MOUSE ; MICE ; PROLIFERATION ; SPERMATOGENESIS ; DISRUPTION ; FERTILITY ; PROTEIN ; REPAIR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Reproductive Biology |
| WOS类目 | Reproductive Biology |
| WOS记录号 | WOS:000813093900001 |
| 出版者 | OXFORD UNIV PRESS INC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ia.ac.cn/handle/173211/49345 |
| 通讯作者 | Hou, Xiaohui; Gao, Fei |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, 1Beichen West Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China 3.Beijing Inst Stem Cell & Regenerat Med, Beijing, Peoples R China 4.Zunyi Med Univ, Dept Cell Biol, 6 Xuefu West Rd, Zunyi 563000, Guizhou, Peoples R China 5.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cen, Changhuo,Chen, Junhua,Lin, Limei,et al. Fancb deficiency causes premature ovarian insufficiency in mice(dagger)[J]. BIOLOGY OF REPRODUCTION,2022:10. |
| APA | Cen, Changhuo.,Chen, Junhua.,Lin, Limei.,Chen, Min.,Dong, Fangfang.,...&Gao, Fei.(2022).Fancb deficiency causes premature ovarian insufficiency in mice(dagger).BIOLOGY OF REPRODUCTION,10. |
| MLA | Cen, Changhuo,et al."Fancb deficiency causes premature ovarian insufficiency in mice(dagger)".BIOLOGY OF REPRODUCTION (2022):10. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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