| CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate | |
Li, Bang1; Zhao, Hui2; Tu, Zhuchi1; Yang, Weili1; Han, Rui1 ; Wang, Lu1; Luo, Xiaopeng1; Pan, Mingtian1; Chen, Xiusheng1; Zhang, Jiawei1; Xu, Huijuan2; Guo, Xiangyu1; Yan, Sen1; Yin, Peng1; Zhao, Zhiguang2; Liu, Jianrong3; Luo, Yafeng3; Li, Yuefeng4; Yang, Zhengyi5; Zhang, Baogui5; Tan, Zhiqiang1; Xu, Hao1; Jiang, Tianzi5; Jiang, Yonghui6; Li, Shihua1; Zhang, Yong Q2; Li, XiaoJiang1
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| 发表期刊 | CELL DISCOVERY
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| ISSN | 2056-5968 |
| 2023 | |
| 卷号 | 9期号:1 |
| 摘要 | Autism spectrum disorder (ASD) is a complex neurodevelopmental condition that affects social interaction and behavior. Mutations in the gene encoding chromodomain helicase DNA-binding protein 8 (CHD8) lead to autism symptoms and macrocephaly by a haploinsufficiency mechanism. However, studies of small animal models showed inconsistent findings about the mechanisms for CHD8 deficiency-mediated autism symptoms and macrocephaly. Using the nonhuman primate as a model system, we found that CRISPR/Cas9-mediated CHD8 mutations in the embryos of cynomolgus monkeys led to increased gliogenesis to cause macrocephaly in cynomolgus monkeys. Disrupting CHD8 in the fetal monkey brain prior to gliogenesis increased the number of glial cells in newborn monkeys. Moreover, knocking down CHD8 via CRISPR/Cas9 in organotypic monkey brain slices from newborn monkeys also enhanced the proliferation of glial cells. Our findings suggest that gliogenesis is critical for brain size in primates and that abnormal gliogenesis may contribute to ASD. |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ia.ac.cn/handle/173211/51796 |
| 专题 | 脑图谱与类脑智能实验室_脑网络组研究 |
| 作者单位 | 1.Jinan University 2.Institute of Genetics & Developmental Biology, CAS 3.Yuanxi Biotech Inc 4.Guangdong Landau Biotechnol Co Ltd 5.Institute of Automation, CAS 6.Yale University |
| 推荐引用方式 GB/T 7714 | Li, Bang,Zhao, Hui,Tu, Zhuchi,et al. CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate[J]. CELL DISCOVERY,2023,9(1). |
| APA | Li, Bang.,Zhao, Hui.,Tu, Zhuchi.,Yang, Weili.,Han, Rui.,...&Li, XiaoJiang.(2023).CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate.CELL DISCOVERY,9(1). |
| MLA | Li, Bang,et al."CHD8 mutations increase gliogenesis to enlarge brain size in the nonhuman primate".CELL DISCOVERY 9.1(2023). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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