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Vertically scanned laser sheet microscopy
Dong, Di1; Arranz, Alicia2; Zhu, Shouping3; Yang, Yujie1; Shi, Liangliang1; Wang, Jun4; Shen, Chen3; Tian, Jie1; Ripoll, Jorge5,6,7; Jie Tian, Jorge Ripoll
AbstractLaser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
KeywordLight Sheet Microscopy Selective Plane Illumination Microscopy Stripe Artifact
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Funding OrganizationBill and Melinda Gates Foundation ; National Basic Research Program of China (973 Program)(2011CB707700) ; National Natural Science Foundation of China(81227901 ; Chinese Academy of Sciences(2010Y2GA03 ; Marie Curie Intra-European Fellowship program(IEF-2010-275137) ; EC FP7 IMI project PREDICT-TB ; EC FP7 CIG grant HIGH-THROUGHPUT TOMO ; Spanish MINECO(FIS2013-41802-R MESO-IMAGING) ; 81027002 ; YZ201164) ; 61231004 ; 81101095)
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000345837200018
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorJie Tian, Jorge Ripoll
Affiliation1.Chinese Acad Sci, Key Lab Mol Imaging, Beijing 100190, Peoples R China
2.ETH, Swiss Fed Inst Technol, Inst Biomed Engn, Zurich, Switzerland
3.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China
4.Harbin Univ Sci & Technol, Sch Automat, Harbin 150080, Peoples R China
5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain
6.Hosp Gen Gregorio Maranon, Inst Invest Sanitaria, Expt Med & Surg Unit, Madrid 28007, Spain
7.Fdn Res & Technol Hellas, Inst Elect Struct & Laser, Iraklion 71110, Greece
Recommended Citation
GB/T 7714
Dong, Di,Arranz, Alicia,Zhu, Shouping,et al. Vertically scanned laser sheet microscopy[J]. JOURNAL OF BIOMEDICAL OPTICS,2014,19(10):106001-8.
APA Dong, Di.,Arranz, Alicia.,Zhu, Shouping.,Yang, Yujie.,Shi, Liangliang.,...&Jie Tian, Jorge Ripoll.(2014).Vertically scanned laser sheet microscopy.JOURNAL OF BIOMEDICAL OPTICS,19(10),106001-8.
MLA Dong, Di,et al."Vertically scanned laser sheet microscopy".JOURNAL OF BIOMEDICAL OPTICS 19.10(2014):106001-8.
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