Institutional Repository of Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Vertically scanned laser sheet microscopy | |
Dong, Di1; Arranz, Alicia2; Zhu, Shouping3; Yang, Yujie1; Shi, Liangliang1; Wang, Jun4; Shen, Chen3; Tian, Jie1; Ripoll, Jorge5,6,7; Jie Tian, Jorge Ripoll | |
发表期刊 | JOURNAL OF BIOMEDICAL OPTICS |
2014-10-01 | |
卷号 | 19期号:10页码:106001-8 |
文章类型 | Article |
摘要 | Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) |
关键词 | Light Sheet Microscopy Selective Plane Illumination Microscopy Stripe Artifact |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1117/1.JBO.19.10.106001 |
关键词[WOS] | PLANE ILLUMINATION MICROSCOPY ; DEVELOPMENTAL BIOLOGY |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Bill and Melinda Gates Foundation ; National Basic Research Program of China (973 Program)(2011CB707700) ; National Natural Science Foundation of China(81227901 ; Chinese Academy of Sciences(2010Y2GA03 ; Marie Curie Intra-European Fellowship program(IEF-2010-275137) ; EC FP7 IMI project PREDICT-TB ; EC FP7 CIG grant HIGH-THROUGHPUT TOMO ; Spanish MINECO(FIS2013-41802-R MESO-IMAGING) ; 81027002 ; YZ201164) ; 61231004 ; 81101095) |
WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
WOS类目 | Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
WOS记录号 | WOS:000345837200018 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ia.ac.cn/handle/173211/11158 |
专题 | 中国科学院分子影像重点实验室 |
通讯作者 | Jie Tian, Jorge Ripoll |
作者单位 | 1.Chinese Acad Sci, Key Lab Mol Imaging, Beijing 100190, Peoples R China 2.ETH, Swiss Fed Inst Technol, Inst Biomed Engn, Zurich, Switzerland 3.Xidian Univ, Sch Life Sci & Technol, Xian 710071, Shaanxi, Peoples R China 4.Harbin Univ Sci & Technol, Sch Automat, Harbin 150080, Peoples R China 5.Univ Carlos III Madrid, Dept Bioengn & Aerosp Engn, Madrid 28911, Spain 6.Hosp Gen Gregorio Maranon, Inst Invest Sanitaria, Expt Med & Surg Unit, Madrid 28007, Spain 7.Fdn Res & Technol Hellas, Inst Elect Struct & Laser, Iraklion 71110, Greece |
推荐引用方式 GB/T 7714 | Dong, Di,Arranz, Alicia,Zhu, Shouping,et al. Vertically scanned laser sheet microscopy[J]. JOURNAL OF BIOMEDICAL OPTICS,2014,19(10):106001-8. |
APA | Dong, Di.,Arranz, Alicia.,Zhu, Shouping.,Yang, Yujie.,Shi, Liangliang.,...&Jie Tian, Jorge Ripoll.(2014).Vertically scanned laser sheet microscopy.JOURNAL OF BIOMEDICAL OPTICS,19(10),106001-8. |
MLA | Dong, Di,et al."Vertically scanned laser sheet microscopy".JOURNAL OF BIOMEDICAL OPTICS 19.10(2014):106001-8. |
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