CASIA OpenIR  > 中国科学院分子影像重点实验室
Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter
Shi, Yaru1; Liu, Wei2; Zheng, Haifeng1; Li, Zhiqiang1; Shi, Xiaorui1; Jiao, Zhiqiang1; Mao, Wenjie1; Xie, Jinrong1; Tian, Jie1,3; Wang, Fu1
Source PublicationBIOMEDICAL OPTICS EXPRESS
2018-02-01
Volume9Issue:2Pages:518-528
SubtypeArticle
AbstractPre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1364/BOE.9.000518
WOS KeywordMAMMALIAN-CELLS ; ANTISENSE OLIGONUCLEOTIDES ; IN-VIVO ; SPLICEOSOME ; EXPRESSION
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010)
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000425923300013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/21955
Collection中国科学院分子影像重点实验室
Affiliation1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Shi, Yaru,Liu, Wei,Zheng, Haifeng,et al. Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter[J]. BIOMEDICAL OPTICS EXPRESS,2018,9(2):518-528.
APA Shi, Yaru.,Liu, Wei.,Zheng, Haifeng.,Li, Zhiqiang.,Shi, Xiaorui.,...&Wang, Fu.(2018).Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter.BIOMEDICAL OPTICS EXPRESS,9(2),518-528.
MLA Shi, Yaru,et al."Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter".BIOMEDICAL OPTICS EXPRESS 9.2(2018):518-528.
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