Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter

doi:10.1364/BOE.9.000518

	Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter
	Shi, Yaru 1; Liu, Wei2 ; Zheng, Haifeng 1; Li, Zhiqiang 1; Shi, Xiaorui 1; Jiao, Zhiqiang 1; Mao, Wenjie 1; Xie, Jinrong 1; Tian, Jie 1,3; Wang, Fu 1
发表期刊	BIOMEDICAL OPTICS EXPRESS
	2018-02-01
卷号	9 期号:2 页码:518-528
文章类型	Article
摘要	Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1364/BOE.9.000518
关键词[WOS]	MAMMALIAN-CELLS ; ANTISENSE OLIGONUCLEOTIDES ; IN-VIVO ; SPLICEOSOME ; EXPRESSION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010)
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000425923300013
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/21955
专题	中国科学院分子影像重点实验室
作者单位	1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Shi, Yaru,Liu, Wei,Zheng, Haifeng,et al. Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter[J]. BIOMEDICAL OPTICS EXPRESS,2018,9(2):518-528.
APA	Shi, Yaru.,Liu, Wei.,Zheng, Haifeng.,Li, Zhiqiang.,Shi, Xiaorui.,...&Wang, Fu.(2018).Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter.BIOMEDICAL OPTICS EXPRESS,9(2),518-528.
MLA	Shi, Yaru,et al."Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter".BIOMEDICAL OPTICS EXPRESS 9.2(2018):518-528.