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Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter
Shi, Yaru1; Liu, Wei2; Zheng, Haifeng1; Li, Zhiqiang1; Shi, Xiaorui1; Jiao, Zhiqiang1; Mao, Wenjie1; Xie, Jinrong1; Tian, Jie1,3; Wang, Fu1
2018-02-01
发表期刊BIOMEDICAL OPTICS EXPRESS
卷号9期号:2页码:518-528
文章类型Article
摘要Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1364/BOE.9.000518
关键词[WOS]MAMMALIAN-CELLS ; ANTISENSE OLIGONUCLEOTIDES ; IN-VIVO ; SPLICEOSOME ; EXPRESSION
收录类别SCI
语种英语
项目资助者National Natural Science Foundation of China(81571721 ; Natural Science Basis Research Plan in Shaanxi Province of China(2016JM8016) ; 81772010)
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号WOS:000425923300013
引用统计
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/21955
专题中国科学院分子影像重点实验室
作者单位1.Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China
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Shi, Yaru,Liu, Wei,Zheng, Haifeng,et al. Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter[J]. BIOMEDICAL OPTICS EXPRESS,2018,9(2):518-528.
APA Shi, Yaru.,Liu, Wei.,Zheng, Haifeng.,Li, Zhiqiang.,Shi, Xiaorui.,...&Wang, Fu.(2018).Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter.BIOMEDICAL OPTICS EXPRESS,9(2),518-528.
MLA Shi, Yaru,et al."Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter".BIOMEDICAL OPTICS EXPRESS 9.2(2018):518-528.
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