Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle

doi:10.1016/j.nano.2018.07.007

	Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle
	Du, Yang1,2,3 ; Fan, Kelong 4; Zhang, Hejun 5; Li, Li 1; Wang, Peixia 3,4; He, Jiuyang 3,4; Ding, Shigang 5; Yan, Xiyun 3,4; Tian, Jie 1,2,3
发表期刊	NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
ISSN	1549-9634
	2018-10-01
卷号	14 期号:7 页码:2259-2270
通讯作者	Ding, Shigang(dingshigang222@163.com) ; Yan, Xiyun(yanxy@sun5.ibp.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
摘要	Optical imaging technologies improve clinical diagnostic accuracy of early gastric cancer (EGC). However, there was a lack of imaging agents exhibiting molecular specificity for EGCs. Here, we employed the dye labeled human heavy-chain ferritin (HFn) as imaging nanoprobe, which recognizes tumor biomarker transferrin receptor 1 (TfR1), to enable specific EGC imaging using confocal laser endomicroscopy (CLE). TfR1 expression was initially examined in vitro in gastric tumor cells and in vivo through whole-body fluorescence and CLE imaging in tumor-bearing mice. Subsequently, dye labeled HFn was topically applied to resected human tissues for EGC detection. CLE analysis of TfR1-targeted fluorescence imaging allowed distinction of neoplastic from non-neoplastic tissues (P < 0.0001), and TfR1 expression level was found to correlate with EGC differentiation degrees (P < 0.0001). Notably, the CLE evaluation correlated well with the immunohistochemical findings (kappa-coefficient: 0.8023). Our HFn-nanoprobe-based CLE increases the accuracy of EGC detection and enables visualization of tumor margins and endoscopic resection. (c) 2018 Elsevier Inc. All rights reserved.
关键词	Early gastric cancer Confocal laser endomicroscopy Human H-ferritin Molecular imaging Fluorescently labeled nanoprobe
DOI	10.1016/j.nano.2018.07.007
关键词[WOS]	IN-VIVO ; CONFOCAL ENDOMICROSCOPY ; PHOTODYNAMIC THERAPY ; DRUG-DELIVERY ; RECEPTOR ; NANOCAGES ; TUMORS
收录类别	SCI
语种	英语
资助项目	Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-SMC013] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDA09030306] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDB02060010] ; Young Elite Scientist Sponsorship Program by CAST[2015QNRC001] ; Research and Development Program of China (973)[2015CB755500] ; National Natural Science Foundation of China[81671810] ; National Natural Science Foundation of China[31530026] ; National Natural Science Foundation of China[81201186] ; National Natural Science Foundation of China[61231004] ; National Natural Science Foundation of China[81571810] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81470083] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2016YFA0201404] ; Ministry of Science and Technology of China[2016YFA0201404] ; Ministry of Science and Technology of China[2017YFA0205200] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81571810] ; National Natural Science Foundation of China[61231004] ; National Natural Science Foundation of China[81201186] ; National Natural Science Foundation of China[31530026] ; National Natural Science Foundation of China[81671810] ; Research and Development Program of China (973)[2015CB755500] ; Young Elite Scientist Sponsorship Program by CAST[2015QNRC001] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDB02060010] ; Strategic Priority Research Program from Chinese Academy of Sciences[XDA09030306] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-SMC013]
项目资助者	Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Research and Development Program of China (973) ; Young Elite Scientist Sponsorship Program by CAST ; Strategic Priority Research Program from Chinese Academy of Sciences ; Key Research Program of Frontier Sciences, CAS
WOS研究方向	Science & Technology - Other Topics ; Research & Experimental Medicine
WOS类目	Nanoscience & Nanotechnology ; Medicine, Research & Experimental
WOS记录号	WOS:000446496900026
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/23047
专题	中国科学院分子影像重点实验室
通讯作者	Ding, Shigang; Yan, Xiyun; Tian, Jie
作者单位	1.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China 2.Beijing Key Lab Mol Imaging, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing, Peoples R China 5.Peking Univ, Hosp 3, Beijing Key Lab Helicobacter Pylori Infect & Uppe, Dept Gastroenterol, Beijing, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所
推荐引用方式 GB/T 7714	Du, Yang,Fan, Kelong,Zhang, Hejun,et al. Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle[J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2018,14(7):2259-2270.
APA	Du, Yang.,Fan, Kelong.,Zhang, Hejun.,Li, Li.,Wang, Peixia.,...&Tian, Jie.(2018).Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,14(7),2259-2270.
MLA	Du, Yang,et al."Endoscopic molecular imaging of early gastric cancer using fluorescently labeled human H-ferritin nanoparticle".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 14.7(2018):2259-2270.