| Intracellular manipulation and measurement with multipole magnetic tweezers | |
Wang, X.1,2 ; Ho, C.1; Tsatskis, Y.3; Law, J.1; Zhang, Z.1; Zhu, M.1,4; Dai, C.1; Wang, F.5; Tan, M.6; Hopyan, S.4,7,8; McNeill, H.3,9; Sun, Y.1,2,10
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| Source Publication | SCIENCE ROBOTICS
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| ISSN | 2470-9476 |
| 2019-03-27 | |
| Volume | 4Issue:28Pages:12 |
| Corresponding Author | Sun, Y.(sun@mie.utoronto.ca) |
| Abstract | The capability to directly interrogate intracellular structures inside a single cell for measurement and manipulation is important for understanding subcellular and suborganelle activities, diagnosing diseases, and developing new therapeutic approaches. Compared with measurements of single cells, physical measurement and manipulation of subcellular structures and organelles remain underexplored. To improve intracellular physical measurement and manipulation, we have developed a multipole magnetic tweezers system for micromanipulation involving submicrometer position control and piconewton force control of a submicrometer magnetic bead inside a single cell for measurement in different locations (spatial) and different time points (temporal). The bead was three-dimensionally positioned in the cell using a generalized predictive controller that addresses the control challenge caused by the low bandwidth of visual feedback from high-resolution confocal imaging. The average positioning error was quantified to be 0.4 mu m, slightly larger than the Brownian motion-imposed constraint (031 mu m). The system is also capable of applying a force up to 60 pN with a resolution of 4 pN for a period of time longer than 30 min. The measurement results revealed that significantly higher stiffness exists in the nucleus' major axis than in the minor axis. This stiffness polarity is likely attributed to the aligned actin filament. We also showed that the nucleus stiffens upon the application of an intracellularly applied force, which can be attributed to the response of structural protein lamin A/C and the intracellular stress fiber actin filaments. |
| DOI | 10.1126/scirobotics.aav6180 |
| WOS Keyword | NUCLEAR MECHANICS ; CELL-NUCLEUS ; DNA-DAMAGE ; FORCE ; MICRO ; SYSTEM ; CHROMATIN ; POLARITY ; REPAIR ; SIZE |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Sciences and Engineering Research Council of Canada ; Canadian Institutes of Health Research via an NSERC Discovery Grant ; Canadian Institutes of Health Research via Collaborative Health Research Projects (CHRP) Grant ; Ontario Research Fund via the Research Excellence Program ; Canada Research Chairs program ; Canadian Institutes of Health Research[143319] ; BJC investigator program ; National Sciences and Engineering Research Council of Canada ; Canadian Institutes of Health Research via an NSERC Discovery Grant ; Canadian Institutes of Health Research via Collaborative Health Research Projects (CHRP) Grant ; Ontario Research Fund via the Research Excellence Program ; Canada Research Chairs program ; Canadian Institutes of Health Research[143319] ; BJC investigator program |
| Funding Organization | National Sciences and Engineering Research Council of Canada ; Canadian Institutes of Health Research via an NSERC Discovery Grant ; Canadian Institutes of Health Research via Collaborative Health Research Projects (CHRP) Grant ; Ontario Research Fund via the Research Excellence Program ; Canada Research Chairs program ; Canadian Institutes of Health Research ; BJC investigator program |
| WOS Research Area | Robotics |
| WOS Subject | Robotics |
| WOS ID | WOS:000464024300004 |
| Publisher | AMER ASSOC ADVANCEMENT SCIENCE |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ia.ac.cn/handle/173211/24915 |
| Collection | 复杂系统管理与控制国家重点实验室_先进机器人 |
| Corresponding Author | Sun, Y. |
| Affiliation | 1.Univ Toronto, Dept Mech & Ind Engn, Toronto, ON M5S 3G8, Canada 2.Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada 3.Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON M5G 1X5, Canada 4.Hosp Sick Children, Program Dev & Stem Cell Biol, Toronto, ON M5G 1X8, Canada 5.HRG Cent Inst Robot, HIT Robot Grp, Harbin 150001, Heilongjiang, Peoples R China 6.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China 7.Hosp Sick Children, Div Orthopaed Surg, Toronto, ON M5G 1X8, Canada 8.Univ Toronto, Toronto, ON M5G 1X8, Canada 9.Washington Univ, Dept Dev Biol, Sch Med, St Louis, MO 63108 USA 10.Univ Toronto, Dept Elect & Comp Engn, Toronto, ON M5S 3G4, Canada |
| Recommended Citation GB/T 7714 | Wang, X.,Ho, C.,Tsatskis, Y.,et al. Intracellular manipulation and measurement with multipole magnetic tweezers[J]. SCIENCE ROBOTICS,2019,4(28):12. |
| APA | Wang, X..,Ho, C..,Tsatskis, Y..,Law, J..,Zhang, Z..,...&Sun, Y..(2019).Intracellular manipulation and measurement with multipole magnetic tweezers.SCIENCE ROBOTICS,4(28),12. |
| MLA | Wang, X.,et al."Intracellular manipulation and measurement with multipole magnetic tweezers".SCIENCE ROBOTICS 4.28(2019):12. |
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