Knowledge Commons of Institute of Automation，CAS
| Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma | |
Hao, He1; Lei, Caiyan2; Dong, QiuLei3 ; Shen, Yalin1; Chi, Jianting1; Ye, Hechun4; Wang, Hong1
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| 发表期刊 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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| 2014-08-01 | |
| 卷号 | 173期号:8页码:2198-2210 |
| 文章类型 | Article |
| 摘要 | The shikonin derivatives, accumulated in the roots of Arnebia euchroma (Boraginaceae), showed antibacterial, anti-inflammatory, and anti-tumor activities. To explore their possible biosynthesis regulation mechanism, this paper investigated the effects of exogenous methyl jasmonate (MJ) on the biosynthesis of shikonin derivatives in callus cultures of A. euchroma. The main results include: Under MJ treatment, the growth of A. euchroma callus cultures was not inhibited, but the expression level of both the genes involved in the biosynthesis of shikonin derivatives and their precursors and the genes responsible for intracellular localization of shikonin derivatives increased significantly in the Red Strain (shikonin derivatives high-producing strain). The quantitative analysis showed that six out of the seven naphthoquinone compounds under investigation increased their contents in the MJ-treated Red Strain, and in particular, the bioactive component acetylshikonin nearly doubled its content in the MJ-treated Red Strain. In addition, it was also observed that the metabolic profiling of naphthoquinone compounds changed significantly after MJ treatment, and the MJ-treated and MJ-untreated strains clearly formed distinct clusters in the score plot of PLS-DA. Our results provide some new insights into the regulation mechanism of the biosynthesis of shikonin derivatives and a possible way to increase the production of naphthoquinone compounds in A. euchroma callus cultures in the future. |
| 关键词 | Arnebia Euchroma Callus Shikonin Derivatives Methyl Jasmonate Gene Expression |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | LITHOSPERMUM CELL-CULTURES ; SUSPENSION-CULTURES ; GENE-EXPRESSION ; ROOT CULTURES ; CDNA CLONING ; ERYTHRORHIZON ; PROTEIN ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000340591800021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ia.ac.cn/handle/173211/2971 |
| 专题 | 多模态人工智能系统全国重点实验室_机器人视觉 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 3.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, He,Lei, Caiyan,Dong, QiuLei,et al. Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2014,173(8):2198-2210. |
| APA | Hao, He.,Lei, Caiyan.,Dong, QiuLei.,Shen, Yalin.,Chi, Jianting.,...&Wang, Hong.(2014).Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,173(8),2198-2210. |
| MLA | Hao, He,et al."Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 173.8(2014):2198-2210. |
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