Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green

doi:10.1038/srep21013

	Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green
	Fang, Cheng 1,2; Wang, Kun2,3 ; Zeng, Chaoting 1; Chi, Chongwei2,3 ; Shang, Wenting2 ; Ye, Jinzuo2 ; Mao, Yamin2 ; Fan, Yingfang 1; Yang, Jian 1; Xiang, Nan 1; Zeng, Ning 1; Zhu, Wen 1; Fang, Chihua 1; Tian, Jie 2,3
发表期刊	SCIENTIFIC REPORTS
	2016-02-11
卷号	6 期号:21013 页码:-
文章类型	Article
摘要	Tissue necrosis commonly accompanies the development of a wide range of serious diseases. Therefore, highly sensitive detection and precise boundary delineation of necrotic tissue via effective imaging techniques are crucial for clinical treatments; however, no imaging modalities have achieved satisfactory results to date. Although fluorescence molecular imaging (FMI) shows potential in this regard, no effective necrosis-avid fluorescent probe has been developed for clinical applications. Here, we demonstrate that indocyanine green (ICG) can achieve high avidity of necrotic tissue owing to its interaction with lipoprotein (LP) and phospholipids. The mechanism was explored at the cellular and molecular levels through a series of in vitro studies. Detection of necrotic tissue and real-time image-guided surgery were successfully achieved in different organs of different animal models with the help of FMI using in house-designed imaging devices. The results indicated that necrotic tissue with a 0.6 mm diameter could be effectively detected with precise boundary definition. We believe that the new discovery and the associated imaging techniques will improve personalized and precise surgery in the near future.
关键词	-
WOS标题词	Science & Technology
DOI	10.1038/srep21013
关键词[WOS]	IN-VIVO ; ACUTE-PANCREATITIS ; CONTRAST AGENTS ; CANCER-SURGERY ; OVARIAN-CANCER ; BREAST-CANCER ; LASER-LIGHT ; ANGIOGRAPHY ; HYPERICIN ; PROTEIN
收录类别	SCI
语种	英语
项目资助者	National Basic Research Program of China (973 Program)(2011CB707700) ; National Natural Science Foundation of China(81227901 ; Open Projects Program of Key Laboratory of Molecular Imaging(Y3SA011x48) ; Equipment research & development project(YZ201164) ; 61231004 ; U1401254)
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000369924700001
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ia.ac.cn/handle/173211/11344
专题	中国科学院分子影像重点实验室
通讯作者	Tian, Jie
作者单位	1.Southern Med Univ, Zhujiang Hosp, Dept Hepatobiliary Surg, Guangzhou 510280, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China 3.Beijing Key Lab Mol Imaging, Beijing 100190, Peoples R China
第一作者单位	中国科学院自动化研究所
通讯作者单位	中国科学院自动化研究所; 中国科学院分子影像重点实验室
推荐引用方式 GB/T 7714	Fang, Cheng,Wang, Kun,Zeng, Chaoting,et al. Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green[J]. SCIENTIFIC REPORTS,2016,6(21013):-.
APA	Fang, Cheng.,Wang, Kun.,Zeng, Chaoting.,Chi, Chongwei.,Shang, Wenting.,...&Tian, Jie.(2016).Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green.SCIENTIFIC REPORTS,6(21013),-.
MLA	Fang, Cheng,et al."Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green".SCIENTIFIC REPORTS 6.21013(2016):-.

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