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Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM
Li, Xixia1,3; Ji, Gang1,3,4; Chen, Xi2; Ding, Wei1,3; Sun, Lei1,3; Xu, Wei1; Han, Hua2; Sun, Fei1,3,4
Source PublicationJOURNAL OF STRUCTURAL BIOLOGY
2017-11-01
Volume200Issue:2Pages:87-96
SubtypeArticle
AbstractLarge scale, high resolution three dimensional (3D) ultrastructural reconstruction of cells and tissues has become increasingly important to our understanding of complex biological systems. There have been a few partial 3D ultra-structures of Caenorhabditis elegans (C. elegans ) reported, however 3D reconstruction of a whole nematode has never been achieved. Here, we independently developed a technique called automatic collector of ultrathin sections scanning electron microscopy and using this methodology, generated a 3D reconstruction of an entire C. elegans larva with 100 nm axial and 15 nm lateral resolution. Compared to the current available ATUM (automated tape-collecting ultramicrotome) technique, our work provides another alternative complete solution that can be applied to obtain large scale 3D ultrastructure of tissues. Our workflow includes an automated hardware system for high throughput serial section collection, a software package for automatic SEM imaging, and an image reconstruction program. These combined techniques can now be used together to rapidly provide access to understand the anatomy of the whole nematodes.
KeywordAutomation Caenorhabditis Elegans Scanning Electron Microscopy 3d Reconstruction Volume Electron Microscopy
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jsb.2017.09.010
WOS KeywordNEMATODE CAENORHABDITIS-ELEGANS ; VOLUME ELECTRON-MICROSCOPY ; C-ELEGANS ; HIGH-RESOLUTION ; NERVOUS-SYSTEM ; FINE-STRUCTURE ; NEURONS ; PRESERVATION ; NEOCORTEX ; BIOLOGY
Indexed BySCI
Language英语
Funding Organizationfoundation of IBP & Leica Microsystems Ltd Joint Laboratory ; Equipment Development&Technology Innovation Programs of the Chinese Academy of Sciences(Y6CZ021001) ; National Natural Science Foundation of China(31501160 ; National Key Foundation for Exploring Scientific Instruments of China(2013YQ12035311) ; 31470838)
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS IDWOS:000416299300002
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ia.ac.cn/handle/173211/21919
Collection类脑智能研究中心_神经计算及脑机交互
Affiliation1.Chinese Acad Sci, Ctr Biol Imaging, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Biophydcs, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Xixia,Ji, Gang,Chen, Xi,et al. Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM[J]. JOURNAL OF STRUCTURAL BIOLOGY,2017,200(2):87-96.
APA Li, Xixia.,Ji, Gang.,Chen, Xi.,Ding, Wei.,Sun, Lei.,...&Sun, Fei.(2017).Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM.JOURNAL OF STRUCTURAL BIOLOGY,200(2),87-96.
MLA Li, Xixia,et al."Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM".JOURNAL OF STRUCTURAL BIOLOGY 200.2(2017):87-96.
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