CASIA OpenIR  > 类脑智能研究中心  > 神经计算及脑机交互
Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM
Li, Xixia1,3; Ji, Gang1,3,4; Chen, Xi2; Ding, Wei1,3; Sun, Lei1,3; Xu, Wei1; Han, Hua2; Sun, Fei1,3,4
2017-11-01
发表期刊JOURNAL OF STRUCTURAL BIOLOGY
卷号200期号:2页码:87-96
文章类型Article
摘要Large scale, high resolution three dimensional (3D) ultrastructural reconstruction of cells and tissues has become increasingly important to our understanding of complex biological systems. There have been a few partial 3D ultra-structures of Caenorhabditis elegans (C. elegans ) reported, however 3D reconstruction of a whole nematode has never been achieved. Here, we independently developed a technique called automatic collector of ultrathin sections scanning electron microscopy and using this methodology, generated a 3D reconstruction of an entire C. elegans larva with 100 nm axial and 15 nm lateral resolution. Compared to the current available ATUM (automated tape-collecting ultramicrotome) technique, our work provides another alternative complete solution that can be applied to obtain large scale 3D ultrastructure of tissues. Our workflow includes an automated hardware system for high throughput serial section collection, a software package for automatic SEM imaging, and an image reconstruction program. These combined techniques can now be used together to rapidly provide access to understand the anatomy of the whole nematodes.
关键词Automation Caenorhabditis Elegans Scanning Electron Microscopy 3d Reconstruction Volume Electron Microscopy
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jsb.2017.09.010
关键词[WOS]NEMATODE CAENORHABDITIS-ELEGANS ; VOLUME ELECTRON-MICROSCOPY ; C-ELEGANS ; HIGH-RESOLUTION ; NERVOUS-SYSTEM ; FINE-STRUCTURE ; NEURONS ; PRESERVATION ; NEOCORTEX ; BIOLOGY
收录类别SCI
语种英语
项目资助者foundation of IBP & Leica Microsystems Ltd Joint Laboratory ; Equipment Development&Technology Innovation Programs of the Chinese Academy of Sciences(Y6CZ021001) ; National Natural Science Foundation of China(31501160 ; National Key Foundation for Exploring Scientific Instruments of China(2013YQ12035311) ; 31470838)
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号WOS:000416299300002
引用统计
文献类型期刊论文
条目标识符http://ir.ia.ac.cn/handle/173211/21919
专题类脑智能研究中心_神经计算及脑机交互
作者单位1.Chinese Acad Sci, Ctr Biol Imaging, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Automat, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Biophydcs, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Xixia,Ji, Gang,Chen, Xi,et al. Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM[J]. JOURNAL OF STRUCTURAL BIOLOGY,2017,200(2):87-96.
APA Li, Xixia.,Ji, Gang.,Chen, Xi.,Ding, Wei.,Sun, Lei.,...&Sun, Fei.(2017).Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM.JOURNAL OF STRUCTURAL BIOLOGY,200(2),87-96.
MLA Li, Xixia,et al."Large scale three-dimensional reconstruction of an entire &ITCaenorhabditis elegans&IT larva using AutoCUTS-SEM".JOURNAL OF STRUCTURAL BIOLOGY 200.2(2017):87-96.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S104784771730(1537KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Xixia]的文章
[Ji, Gang]的文章
[Chen, Xi]的文章
百度学术
百度学术中相似的文章
[Li, Xixia]的文章
[Ji, Gang]的文章
[Chen, Xi]的文章
必应学术
必应学术中相似的文章
[Li, Xixia]的文章
[Ji, Gang]的文章
[Chen, Xi]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S1047847717301612-main (2).pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。