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A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes
Johnson, Wesley1; Dai, Changsheng1; Liu, Jun; Wang, Xian1; Luu, Devin K.1; Zhang, Zhuoran1; Ru, Changhai2,3; Zhou, Chao4; Tan, Min; Pu, Huayan5; Xie, Shaorong5; Peng, Yan5; Luo, Jun5; Sun, Yu1,5
AbstractMammalian oocytes such as mouse oocytes have a highly elastic outer membrane, zona pellucida (ZP) that cannot be penetrated without significantly deforming the oocyte, even with a sharp micropipette. Piezo drill devices leverage lateral and axial vibration of the micropipette to accomplish ZP penetration with greatly reduced oocyte deformation. However, existing piezo drills all rely on a large lateral micropipette vibration amplitude (> 20 mu m) and a small axial vibration amplitude (< 0.1 mu m). The very large lateral vibration amplitude has been deemed to be necessary for ZP penetration although it also induces larger oocyte deformation and more oocyte damage. This paper reports on a new piezo drill device that uses a flexure guidance mechanism and a systematically designed pulse train with an appropriate base frequency. Both simulation and experimental results demonstrate that a small lateral vibration amplitude (e.g., 2 mu m) and an axial vibration amplitude as large as 1.2 mu m were achieved. Besides achieving 100% effectiveness in the penetration of mouse oocytes (n = 45), the new piezo device during ZP penetration induced a small oocyte deformation of 3.4 mu m versus larger than 10 mu m using existing piezo drill devices.
KeywordCell Manipulation Piezo Drill Zona Pellucida Penetration
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Funding OrganizationNatural Sciences and Engineering Research Council of Canada ; Canada Research Chairs Program ; Ontario Research Fund-Research Excellence ; National Natural Science Foundation of China(61528304 ; Shanghai Municipal Science and Technology Commission Project(14JC1491500) ; 61525305 ; 61403245)
WOS Research AreaEngineering
WOS SubjectEngineering, Biomedical
WOS IDWOS:000425664500021
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Univ Toronto, Dept Mech & Ind Engn, Toronto, ON M5S 3G8, Canada
2.Soochow Univ, Jiangsu Prov Key Lab Adv Robot, Suzhou, Peoples R China
3.Soochow Univ, Collaborat Innovat Ctr Suzhou Nano Sci & Technol, Suzhou, Peoples R China
4.Chinese Acad Sci, Inst Automat, Beijing, Peoples R China
5.Shanghai Univ, Sch Mech Engn & Automat, Shanghai 200072, Peoples R China
Recommended Citation
GB/T 7714
Johnson, Wesley,Dai, Changsheng,Liu, Jun,et al. A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes[J]. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING,2018,65(3):678-686.
APA Johnson, Wesley.,Dai, Changsheng.,Liu, Jun.,Wang, Xian.,Luu, Devin K..,...&Sun, Yu.(2018).A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes.IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING,65(3),678-686.
MLA Johnson, Wesley,et al."A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes".IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING 65.3(2018):678-686.
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